The Imaging / Histology Core supports the use and maintenance of instrumentation for imaging of live and preserved organs, cells, tissues, as well as plates and arrays. This Core also provides computer software for the processing and quantification of image features.
The Imaging Core is supported by NEI Core Center Grant P30 EY014801. Please cite this grant number on all publications that make use of this core.
Leadership & Management
Valery Shestopalov, Ph.D. directs the Imaging/Histology Module. Dr. Shestopalov is a Research Professor of Ophthalmology. Mr. Charles Yaros, Senior Manager of Research Support, manages the Imaging/Histology Core on a daily basis as well as assisting researchers with their imaging and image analysis needs.
Resources & Location
The core is comprised of three different facilities located on different floors of the McKnight Vision Research Center building:
Please click on the links above to go directly to information about each facility. Please contact Mr. Charles Yaros for access to the University Sharepoint Website with instrument schedules.
- Leica Stellaris 5 Inverted Confocal Microscope
- Leica DMI 6000 CS inverted Microscope
The Histology portion of this Core supports the preparation, embedding, sectioning, and storage of biological specimens for observation and analysis by light microscopy. This is accomplished vid the provision of Training for users and instrument availability allowing users to reserve instruments for the preparation of their specimens.
The Core provides fresh, frozen, and paraffin specimen processing. Equipment for routine staining is available, including H&E, PAS, and MT.
Specimens can be examined at all stages of preparation by standard light microscopy and phase contrast microscopy.
The Histology portion of the Core is located on the 4th floor of the McKnight building in room 409. The lab was carefully designed for efficient work flow. It contains a built-in Labconco fume hood (with blower, cabinet and work surface for staining apparatus), and 2 chemical sinks.
Equipment Located in the Histology Lab
- ATP™ Tissue Processor (# ATP1-120, Triangle Biomedical Sciences, Inc.)
- 2 Microtome Cryostats (International Equipment Company – Minotome)
- Leica Jung knife sharpener
- 2 micro optics Microtomy (Leitz #1512)
- Spencer binocular microscope (AO #926963)
- Lancer Vibratome 1000 and Vibratome 1000 Plus
- Illuminated Tissue Flotation Bath (# 80086-990, VWR)
- Paraffin Dispenser (# PD-120, Triangle Biomedical Sciences, Inc)
For more information, please contact:Mr. Charles Yaros
Senior Manager of Research Support, Imaging Core Manager
McKnight Vision Research Center, 5th Floor