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Stereo Microscopy

The Stereo Microscopy Facility supports the use and maintenance of microscopy instrumentation for stereo imaging of cells, tissues, and organs. Macroscopic items like whole organs, and whole animals thru both white and fluorescent light. As well as provide good visibility for animal surgeries. GFP-transgenic, and other fluorescently labeled cells and tissues, can be viewed, manipulated, and imaged using these instruments.

Location

The Stereo microscopy Facility is located on the 6th floor of the McKnight building, room 621

Equipment

  • Zeiss Stemi SV11 Fluorescence Stereo Microscope with AxioCam Mrc Camera. This microscope is equipped with 1.6x high optical quality lenses to provide wide-field stereoscopic image. H-50 arc lamp provides adequate source of light to illuminate fluorescence-labeled samples. Two different excitation/ emission (DAPI, FITC) filter pairs allow multiple fluorophores to be visualized and/or captured by the digital camera.
  • GE imageQuant LAS400 imaging station with CCD camera

Location

The wide-field fluorescence microscopy Facility is also located in room 621

Equipment

  • Axiostar Plus Upright Fluorescence Microscope
  • AxioCam Mrc Camera

This research grade microscope is equipped with 5x, 10x, 20x, and 40x objectives. Three different excitation/ emission (DAPI, FITC, CY3) filter pairs allow multiple fluorophores to be visualized and/or captured by the digital camera and processed with analytical software.