Goals and Purpose
The purpose of this grant program is to provide investigators with up to $10,000 to purchase reagents to develop preliminary data using mass cytometry or mass imaging cytometry.
The FCSR has recently installed new instrumentation for Mass Cytometry with the acquisition of a Fluidigm Helios cytometer (Mass Cytometry, ‘CYTOF’), and Fluidigm Hyperion tissue imager (Mass Imaging Cytometry). The Helios allows users to perform highly multiplexed experiments of single cells in suspension (similar to flow cytometry), with 50+ parameters possible simultaneously. This is accomplished by using unique metal isotope labeled antibodies. The cells are then ionized and the time of flight for the unique metals to reach the detector allow for the quantitation of antibodies on each cell. The Hyperion module allows for imaging of tissue sections labeled with metal antibodies by ionizing the tissue 1 micron2 at a time and sending the ion cloud to the Hyperion for quantification. To read more about these technologies, please go to https://www.fluidigm.com/reagents/mass-cytometry for information on Helios, and to https://www.fluidigm.com/applications/imaging-mass-cytometry for information on the technology and what reagents are currently available.
Description of Award Mechanism
Each award is up to $10,000
Applications are restricted to Cancer Center Members (faculty) only. Graduate Students and Post-Docs in members’ laboratories are not eligible.
Only cancer-focused research projects will be considered in the award process.
Tuesday, April 30, 2019