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Flow Cytometry Forms

To access the Flow Cytometry Shared Resource’s services, please review and complete the forms below, as appropriate. Use of the Flow Cytometry Shared Resource requires familiarization with the published policies and procedures. A Billing Authorization Form is required for use of the Flow Cytometry Shared Resource, and must be resubmitted monthly. All Principal Investigators are required to submit a current Biohazard Risk Assessment form annually. In addition, each new cell sort performed within the facility requires prior submission of a completed risk assessment form for all Arias.

Procedures
Policies and Procedures
Flow Cytometry Core Billing Authorization Form

iLab online instrument reservation system
umiami.corefacilities.org

Instructions on how to register for an iLab account
Instructions on how to make a reservation using iLab

Free Cytospec data analysis software
www.cyto.purdue.edu/Purdue_software

Flow Cytometry ListServ (for exchange of information between facility users and staff)
Join or access this ListServ

Application and technical notes

Bio-Medical Applications of Flow Cytometry
Analysis of Nucleic Acids Using Flow Cytometry
BD multi-color flow cytometry online resource tools

External links

Purdue University Flow Cytometry Laboratories (an excellent resource)
http://www.cyto.purdue.edu

Purdue University Cytometry Mailing List (for all cytometry-related inquiries and a wealth of shared information)
http://www.cyto.purdue.edu/hmarchiv/index.htm

Education and training
http://www.cyto.purdue.edu/flowcyt/educate/pptslide.htm
http://www.cyto.purdue.edu/class/
http://www.cyto.purdue.edu/flowcyt/labinfo/labinfo.htm

Becton Dickinson Immuno-cytometry Systems
http://www.bdbiosciences.com/us/home/

International Society for Analytical Cytology
http://www.isac-net.org

Molecular Probes (reagents)
https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook.html

Beckman-Coulter
https://www.beckmancoulter.com/

Beckman-Coulter cytometry site
https://www.beckman.com/coulter-flow-cytometers

Free cytometry software downloads
http://www.cyto.purdue.edu/flowcyt/software/Catalog.htm

Download the seminal work: “Practical Flow Cytometry” fourth edition, by Howard Shapiro
https://www.beckman.com/resources/reading-material/ebooks/practical-flow-cytometry

Download Flow-Cytometry-A Basic Introduction, by Michael Ormerod
http://www.denovosoftware.com/site/introtoflowormerod.shtml

Fluorescence spectrum viewers and tables
http://www.bdbiosciences.com/eu/s/spectrumviewer
http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Fluorescence-SpectraViewer.html https://www.thermofisher.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html

Treestar (FlowJo) software
http://www.flowjo.com

Online FlowJo tutorial page
https://www.flowjo.com/solutions/flowjo/downloads/tutorials

DeNovo (FCS Express) software
https://www.denovosoftware.com/

Verity software
http://www.vsh.com/

Cytek Biosciences
http://www.cytekbio.com/

Thermofisher (Affymetrix)
http://www.thermofisher.com/

Fluidigm (CyTOF)
http://www.fluidigm.com/
https://www.fluidigm.com/applications/imaging-mass-cytometry

Miltenyi Biotechnology
http://www.miltenyibiotec.com/

BD online customer training site (contains interactive courses covering an introduction to flow cytometry, DiVa software, Canto-II, cell-cycle analysis, etc.)
http://www.bdbiosciences.com/eu/support/s/training

Free online repository for flow cytometry data, including complementary data analysis software, CytoBank
www.cytobank.org

The new International Cytometry Certification Examination (ICCE) online applications
www.cytometrycertification.org

The Flow Cytometry Network (FCN): FCN allows you to manage your profile and interact with collaborators, through free video conferencing, messaging and more. Create and feature lab groups on FCN. Access free flow cytometry experimental design, data analysis and other software tools. Upload and share cytometry based protocols within the community. Get real time notifications on forum discussions (on topics of interest) by email and/or FCN profile. Keep updated with the latest flow cytometry research and industry news. Plan, post, and RSVP to events and meetings. Post and search for jobs. Access free research articles as available.
http://www.thefcn.com

Panel design web-sites
http://www.fluorofinder.com
https://www.biolegend.com/panelselector
https://www.chromocyte.com/calculate