Principal Investigator
Enrollment Status
Clinical Trial ID
Clinical Trial Summary
This study is an open-label, Phase 1, multicenter study to evaluate the safety, tolerability,
pharmacokinetics (PK), and pharmacodynamic (PD) profiles of a novel fragment crystallizable
(Fc)-engineered immunoglobulin G1 anti-cytotoxic T-lymphocyte antigen 4 (anti-CTLA-4) human
monoclonal antibody (botensilimab) monotherapy and in combination with an anti-programmed
cell death protein-1 (PD-1) antibody (balstilimab), and to assess the maximum tolerated dose
(MTD) in participants with advanced solid tumors. This study will also determine the
recommended phase 2 dose (RP2D) of botensilimab monotherapy and in combination with
balstilimab.
Phase
Phase 1
Funding Agency/Sponsor
Industrial
Disease
Bone and Soft Tissue Cancers
Enrollment Eligibility
Inclusion Criteria:
For inclusion in the trial, all of the following inclusion criteria must be fulfilled, as
no waivers will be permitted:
1. Provision of signed and dated written informed consent prior to any study specific
procedures. Participation in pharmacogenomics testing is optional.
2. Histologically or cytologically confirmed diagnosis of metastatic or locally advanced
solid tumor for which no standard therapy is available or standard therapy has failed.
3. Measurable disease on imaging based on RECIST 1.1, except for prostate cancer.
4. Life expectancy of ≥ 3 months and Eastern Cooperative Oncology Group performance
status of 0 or 1.
5. Adequate organ and bone marrow reserve function, as indicated by the following
laboratory values:
1. Adequate hematological function, defined as absolute neutrophil count ≥ 1.5 ×
10^9/liter (L), platelet count ≥ 100 × 10^9/L, and hemoglobin ≥ 8 grams/deciliter
without recent transfusion (defined as a transfusion that has occurred within 2
weeks of the hemoglobin measurement).
2. Adequate liver function, defined as total bilirubin level ≤ 1.5 × institutional
upper limit of normal (IULN) (except for participants with Gilbert syndrome who
must have a total bilirubin level of ≤ 3.0 × IULN), aspartate aminotransferase ≤
2.5 × IULN, and alanine aminotransferase ≤ 2.5 × IULN.
3. Adequate renal function defined as creatinine ≤ 1.5 × IULN or measured or
calculated creatinine clearance ≥ 40 milliliters (mL)/minute per institutional
standard. Assessment methods should be recorded.
4. Adequate coagulation, defined as international normalized ratio or prothrombin
time ≤ 1.5 × IULN and activated partial thromboplastin time ≤ 1.5 × IULN (unless
participant receiving anticoagulant therapy) or stable known coagulopathy with
sponsor approval.
6. No history of prior or concomitant malignancy that requires other active treatment.
7. A sufficient and adequate formalin-fixed paraffin embedded tumor tissue sample (fresh
or archival tumor tissue) collected since last treatment and before the first dose
from a site not previously irradiated, if clinically feasible.
8. Female participants of childbearing potential must have a negative serum pregnancy
test at screening (within 72 hours of first dose of study medication).
Non-childbearing potential is defined as 1 of the following:
1. ≥ 45 years of age and has not had menses for > 1 year.
2. Amenorrheic for > 2 years without a hysterectomy and/or oophorectomy and follicle
stimulating hormone value in the postmenopausal range upon pretrial (screening)
evaluation.
3. Status is post-hysterectomy, -oophorectomy, or -tubal ligation.
9. Female participants of childbearing potential must be willing to use highly effective
contraceptive measures starting with the Screening visit through 90 days after last
dose of study treatment. Note: Abstinence is acceptable if this is the established and
preferred contraception for the participant.
10. Male participants with a female partner(s) of childbearing potential must agree to use
highly effective contraceptive measures throughout the trial starting with the
Screening visit through 90 days after the last dose of study treatment is received.
Males with pregnant partners must agree to use a condom; no additional method of
contraception is required for the pregnant partner. Note: Abstinence is acceptable if
this is the established and preferred contraception method for the participant.
The following inclusion criteria are in addition to the above criteria. If there are
criteria below that differs from above, the below indication-specific criteria take
precedence.
Additional Inclusion Criteria for Angiosarcoma Cohort
11. Histologically or cytologically confirmed diagnosis of metastatic or locally advanced
angiosarcoma for which no standard therapy is available or standard therapy has
failed.
Additional Inclusion Criteria for the Hepatocellular Cancer (HCC) Cohort
12. Histologically or cytologically confirmed diagnosis or radiological diagnosis
following the guidelines from the American Association for the Study of Liver Diseases
of metastatic or locally advanced HCC.
13. Must have progressed while receiving, or following, programmed death-ligand 1
(PD(L)-1)-based therapy.
14. Child-Pugh score of A. Note: Participants on anticoagulant treatment would have an
assigned value of 1 point when scoring prothrombin time/international normalized ratio
so the overall Child-Pugh score is not adversely affected.
15. Adequate organ and bone marrow reserve function as indicated by the following
laboratory values:
1. Platelet count ≥ 60 × 10^6/cubic millimeter (mm^3) and absolute neutrophil count
≥ 1,000 × 10^6/L are acceptable provided that the investigator assesses these
abnormalities as being due to liver disease.
2. Adequate liver function, defined as aspartate aminotransferase and alanine
aminotransferase ≤ 5 × IULN, bilirubin ≤ 2 × IULN.
16. Participants are eligible to enroll if they have non-viral-HCC or if they have
hepatitis B (HBV), or hepatitis C virus (HCV) related HCC, defined as follows:
1. Chronic HBV infection as evidenced by detectable HBV surface antigen or HBV DNA.
Participants with chronic HBV infection must be on antiviral therapy and have HBV
DNA < 500 international units/mL.
2. Active or resolved HCV infection as evidenced by detectable HCV RNA or antibody.
Additional Inclusion Criteria for the Non-Small Cell Lung Cancer (NSCLC) Cohort
17. Histologically or cytologically confirmed diagnosis of metastatic or locally advanced
NSCLC for which no standard therapy is available or standard therapy has failed:
1. Adenocarcinoma or squamous cell carcinoma at the time of enrollment. If other
histologies are also present, must be approved by the medical monitor prior to
study entry.
2. For participants without targetable alterations: Prior treatment with anti
PD(L)-1-based therapy.
3. Participants with targetable alterations (for example, estimated glomerular
filtration rate, anaplastic lymphoma kinase, Kirsten rat sarcoma virus-single
point mutation with a glycine-to-cysteine substitution at codon 12, reactive
oxygen species, mesenchymal epithelial transition factor receptor, etc.): must
have received or be intolerant of at least one approved targeted therapy.
Additional Inclusion Criteria for the Prostate Cancer Cohort
18. Diagnosis of metastatic castrate resistant prostate cancer.
19. Must have demonstrated serologic or radiographic progression on or following the most
recent therapy in the setting of castrate-level testosterone (< 50 nanograms per mL
[ng/mL] and/or maintained on medical/surgical castration throughout) as defined by at
least one of the following:
1. Baseline PSA ≥ 2.0 ng/mL and 2 sequential rises in prostate-specific antigen
(PSA) with each rising value being at least 1 week apart.
2. Progression by RECIST 1.1.
3. Progression by PCWG3 criteria for bone disease ("2+2" rule) with or without PSA
progression.
20. Must maintain castration status defined as serum testosterone < 50 ng/mL. Must be
either surgically castrate or on luteinizing hormone-releasing hormone analog for the
duration of the study.
Additional Inclusion Criteria for Breast Cancer
21. Must have received PD-(L)1 therapy if indicated. Note: Premenopausal participants may
continue ongoing ovarian suppression on study. Permitted agents are goserelin,
triptorelin or analogs.
Exclusion Criteria:
For inclusion in the trial, participant must meet none of the following exclusion criteria,
as no waivers will be permitted:
1. Currently participating and receiving study therapy or has participated in a study of
an investigational agent and received study therapy or used an investigation device
within 3 weeks of first dose of current study drug.
2. Received prior systemic cytotoxic chemotherapy, biological therapy, radiotherapy, or
major surgery within 3 weeks prior to first dose of study drug; for tyrosine kinase
inhibitor or similar within 4 × half-life prior to first dose of study drug. A 1-week
washout is permitted for palliative radiation to non-central nervous system disease,
with Sponsor approval.
3. Participants who have received prior CTLA-4 therapy may be enrolled in selected
indications upon agreement with the Sponsor.
4. Persistent toxicity of NCI CTCAE version 5.0 Grade > 1 severity that is related to
prior therapy. Note: Sensory neuropathy, hypothyroidism or alopecia of Grade ≤ 2 are
acceptable. Other Grade 2 toxicities of prior treatments that are controlled with
medication (for example, diabetes or hypertension) may be permitted with sponsor
approval.
5. Expected to require any other form of systemic or localized antineoplastic therapy
while on trial (including maintenance therapy with another agent, radiation therapy,
and/or surgical resection).
6. History of:
1. Severe (Grade ≥ 3) hypersensitivity reaction to a fully human monoclonal
antibodies
2. Immune-related adverse event requiring treatment with systemic steroids for > 7
days excluding Grade 1 or 2 rash.
3. Interstitial lung disease or lung disease which may interfere with the assessment
of pneumonitis.
4. Uncontrolled asthma (that is, ≥ 3 features of partly controlled asthma)
5. Pneumonitis that has required oral or IV corticosteroids.
7. Receiving systemic corticosteroid therapy 1 week prior to the first dose of study drug
or receiving any other form of systemic immunosuppressive medication. Note:
Corticosteroid use as a premedication for IV contrast allergies/reactions is allowed.
Participants who are receiving daily corticosteroid replacement therapy are also an
exception to this rule. Daily prednisone at doses of ≤ 7.5 mg or equivalent
hydrocortisone dose are examples of permitted replacement therapy. Use of inhaled or
topical corticosteroids is permitted.
8. Brain metastases or leptomeningeal metastases with the following exceptions: Note:
Brain metastases which have been treated with either surgical resection or
stereotactic radio surgery. These participants must be off steroids ≥ 10 days prior to
randomization for the purpose of managing their brain metastases. Repeat brain imaging
following surgical resection or stereotactic radiosurgery is not required if their
last brain magnetic resonance imaging is within screening window. Prior whole-brain
radiation may be permitted only with sponsor approval. Note: Untreated isolated brain
metastases that are too small for treatment by surgical resection or stereotactic
radiosurgery (for example, 1-2 mm) and/or of uncertain etiology are potentially
eligible but must be approved by the sponsor.
9. Active or history of autoimmune disease that requires systemic treatment within 2
years of the start of study drug (that is, with use of disease-modifying agents,
corticosteroids, or immunosuppressive drugs). Note: Participants with autoimmune
conditions requiring hormone replacement therapy or topical treatments are eligible.
10. Has had an allogeneic tissue/solid organ transplant, except for corneal transplants.
11. Active infection requiring systemic treatment.
12. Known history of human immunodeficiency virus type 1 or 2 antibodies.
13. Known active infection with hepatitis B and/or hepatitis C virus.
14. Clinically significant (that is, active) cardiovascular disease: cerebral vascular
accident/stroke or myocardial infarction within 6 months of enrollment, unstable
angina, congestive heart failure (New York Heart Association class ≥ II), or serious
uncontrolled cardiac arrhythmia requiring medication.
15. History or current evidence of any condition, therapy, any active infections, or
laboratory abnormality that might confound the results of the trial, interfere with
the participant's participation for the full duration of the trial, or is not in the
best interest of the participant to participate, in the opinion of the treating
Investigator.
16. Known psychiatric or substance abuse disorder that would interfere with cooperation
with the requirements of the study.
17. Legally incapacitated or has limited legal capacity.
18. Pregnant or breastfeeding.
19. Concurrent malignancy requiring treatment or history of prior malignancy active within
2 years prior to the first dose of study treatment. Exceptions: participants with
completely resected prior early-stage basal/squamous cell skin cancer or treated
cervical carcinoma in situ.
The following exclusion criteria are in addition to the above criteria. If there are
criteria below that differs from above, the below indication-specific criteria take
precedence.
Additional Exclusion Criteria for the HCC Cohort
20. Received locoregional therapy (for example, transcatheter chemoembolization,
radiation, surgery) within 6 weeks or yttrium-90 within 12 weeks.
21. Hepatic encephalopathy within the last 6 months requiring admission or initiation of
or intensification of therapy. Participants taking rifaximin/lactulose as
encephalopathy prophylaxis are allowed as long as they have not had clinically evident
encephalopathy in the past 6 months.
22. Gastro-esophageal varices bleeding in the last 6 months.
23. Ascites requiring paracentesis within the last 3 months. Participants with previous
ascites that is managed with stable doses of diuretics and have a Child Pugh score of
A are allowed.