The Imaging Core supports the use and maintenance of a Leica DMI 6000B CS inverted microscope system for in vivo fluorescence microscopy. This microscope provides a wide range of capabilities for imaging live cell dynamics.

Location

This microscope is located on the 6th floor of the McKnight building, room 621.

Equipment

• Leica DMI 6000B CS Microscope
• Leica DFC 7000 C Camera
• Five objectives (10x, 20x, 40x, dry and wet, 63x wet)
• Fluorescent filters for GFP, DAPI 390 and Y5HP
• HP Computer System running Leica LAS X Software designed for fluorescence quantitative multiplex and time-lapse imaging. This software has a wide range of image processing tools including Edit, Adjust, Lightening, Deconvolution Noise Reduction, Segmentation, Dye Separation, and Topological modifications and stitching together of multiple images.
• A Controlled Atmosphere Chamber surrounds the microscope allowing live specimen to be monitored and time-lapse imaged over prolonged periods under optimal conditions