The OGSR is located on the 4th floor of the Papanicolaou Cancer Research Building (PAP) and occupies 2,467 sq.ft. of laboratory space and offices. It comprises distinct pre- and post-PCR labs and BSL2 facilities to support rigorous workflows.
10X Genomics
Illumina NextSeq 500 Sequencers
Nano String Gen2 system
Perkin Elmer Zephyr G3 NGS liquid handler PAP 428
OGSR Major Instrumentation:
| Instrument | Description |
|---|---|
| Illumina NovaSeq X+ | Production scale, short read NGS sequencer |
| Illumina NovaSeq 6000 | Production scale, short read NGS sequencer |
| Illumina NextSeq 500 | Rapid run, benchtop, short read NGS sequencer |
| Illumina iSeq 100 | Low output benchtop, short read NGS sequencer |
| PE Zephyr G3 NGS | Automated liquid handler for NGS library prep |
| Hamilton Vantage | Automated liquid handler for NGS library prep |
| Thermo Kingfisher APEX | Automated 24/96 mag-bead extraction system |
| QIAgen QIAcube | Automated 12 tube column extraction system |
| UVP UV3 HEPA cabs (two) | HEPA filtered PCR workstations with UV air irradiation |
| BSL2 safety cabinet (two) | HEPA filtered circulating air safety cabinets |
| 10x Genomics Chromium X | Single cell controller for genomics assays |
| Mission Bio Tapestri | Single cell controller for genomics assays |
| Nexcelom K2 | Imaging cell counter with 2 channel fluorescence |
| 10X Genomics CytAssist | Tissue section transfer and imaging for Visium spatial multiomics |
| nanoString GeoMx | Digital spatial profiler (DSP) for spatial multiomics |
| nanoString Gen2 | nanoString PrepStation and nCounter scanner (2nd generation) |
| Agilent 5200 | 12 capillary DNA/RNA fragment analyzer |
| Agilent 2100 | Lab-on-chip DNA/RNA fragment analyzer |
| Thermo Qubit (two) | Single tube fluorimeter for dye-based DNA/RNA quantification |
| BioTek Synergy H1 | Microplate reader, multi-channel fluorescence |
| Bio-Rad C1000 (five) | Thermal cyclers with deep-well and standard blocks |
| Bio-Rad CFX96 | Multichannel real-time PCR machine |
| QIAgen QIAcuity One | Nano-plate digital PCR system, 24/96 well, 5 channels |
| Eppendorf MasterCycler S | Thermal cycler, 96-well with vapor-protect lid |
| Thermo NanoDrop 8000 | Micro-volume droplet spectrophotometer |