The Cancer Proteomics Shared Resource offers a range of proteomics services designed to address diverse cancer research questions, from protein identification to global signaling analysis.
Gel Band Protein Identification
Protein identification from excised gel bands with high‑confidence peptide sequencing. Workflows can include analysis of post‑translational modifications (PTMs).
Cross‑Linking Mass Spectrometry
Mass spectrometry (MS) cleavable cross‑linking workflows (e.g., using disuccinimidyl sulfoxide (DSSO), an amine-reactive, MS-cleavable cross-linker) to study protein-protein interactions and structural proximity within protein complexes, supported by advanced data analysis pipelines.
Immunoprecipitation and Pulldown Proteomics
Quantitative analysis of protein-protein interactions using immunoprecipitation or affinity‑based pulldown strategies. Experiments may be performed using tandem mass tag (TMT)‑based multiplexing or label‑free data-independent acquisition (DIA)/data-dependent acquisition (DDA) approaches.
Global Proteomics and PTM Analysis
Comprehensive protein quantitation from whole‑cell lysates using TMT or DIA workflows. The CPSR also supports global analysis of post‑translational modifications, including phosphorylation, and acetylation, to interrogate signaling pathways and regulatory mechanisms.
Open Consultation Hours
CPSR offers weekly Open Consultation Hours for investigators who would like to discuss potential projects, experimental design considerations, or workflow selection. These sessions provide an opportunity to connect directly with the CPSR leaders prior to submitting a service request and help ensure that projects are well matched to available proteomics workflows.
• When: Tuesdays, 11:00 am - 1:00 pm
• Format: In‑person, first‑come/first‑served
• Location: Gautier Building, Conference Room 127
• Appointments: Not required
Requesting Services
All CPSR services are requested and managed through iLab. Investigators are encouraged to attend Open Consultation Hours when planning new studies, but formal service requests must be submitted through iLab before samples are accepted.