Skip to Main Content

Flow Cytometry Shared Resource

The Flow Cytometry Shared Resource (FCSR) at Sylvester provides researchers with sophisticated methods for the analysis and preparative sorting of both normal and tumor cells. The facility helps researchers to measure apoptosis, gene expression, drug metabolism, immune responses, and pathways of cellular activation, both normal and tumor-related.

The shared resource maintains instruments, computer workstations for data analysis, and related equipment, to support scientists affiliated with Sylvester’s multidisciplinary research programs, as well as researchers in a wide variety of cancer-related research projects.

The facility also provides training to investigators in flow cytometric applications and cell sorting technologies.

Developments

In order to improve continually, the Flow Cytometry Shared Resource and the services offered, the following areas are under consideration and/or development:

The entire facility was recently relocated to a significantly larger and purpose-built new space on the seventh floor of the Rosenstiel building, from the previous location on the third floor.

Modernization of technology, and replacement of aging, unreliable instruments. For instance, the installation of a new custom-built (SORP) FACS Aria Fusion, four-laser cell sorter. Also, a recent Bankhead-Coley shared instrument grant-funded installation of a special order (SORP) custom-built LSR-Fortessa-HTS, five-laser analytical flow cytometer. The LSR-II has also received a SORP performance enhancement upgrade, replacing an unserviceable old U.V. laser with a new custom-built red + green twin laser engine, and further fluorescence detectors were recently added. The antiquated LSR-I analyzer was replaced with a new, three-laser FACS Canto-II instrument. The old Aria-I cell sorter was replaced with a previously shared SORP Aria-II. The MoFlo XDP sorter was taken out of service. All of the computer workstations have been upgraded and now run the DiVa-8 software.

Online and software enhancements, including our web-based scheduling system (now migrated over to the iLabs software suite), FlowJo data analysis software and ModFit-LT DNA cell-cycle analysis software. The addition of FCS Express data analysis software is also being considered.

The use of our trained personnel, to provide on-demand, extended hours of operation, with staggered shifts as required, has been implemented.

  • A dedicated central computer server has been provided, for off-line storage, analysis and archiving of data.
  • Promoting greater use of the available technology, such as using extended combinations of multiple fluorochrome panels, BSL-2 sorting, flow cloning, etc.
  • Keeping abreast of new technology in the areas of instrumentation, software, fluorescent dyes, and other applications, e.g. by sponsoring appropriate flow cytometry presentations by leaders in the field.

Continually informing researchers of any new information, protocols and applications, via our regular seminar and workshop series, regular e-mail updates, postings of such information on the FCSR’s section of Sylvester’s website, and announcements in the iLab online instrument reservation system.

  • Providing further training in flow cytometer use and data analysis, to promote independent use of the shared resource’s instruments, as well as to facilitate better user application of flow cytometry data.

Contact us

Huw S. (“Zip”) Kruger Gray, Ph.D., Director
Email: HKGray@med.miami.edu

Phone

305-243-5571

Mailing Address
Rosenstiel Medical Sciences Building
1600 N.W. 10th Avenue
Room 7147, Locator R-127
Miami, FL 33136

Physical Address
Rosenstiel Medical Sciences Building
1600 N.W. 10th Avenue
Room 7147
Miami, FL 33136