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Flow Cytometry Shared Resource

The Flow Cytometry Shared Resource (FCSR) at Sylvester provides researchers with sophisticated methods for the analysis and preparative sorting of both normal and tumor cells. The facility helps researchers to measure parameters including apoptosis, gene expression, drug metabolism, immune responses and pathways of cellular activation, both normal and tumor-related.

The shared resource maintains instruments, computer workstations for data analysis and related equipment, to support scientists affiliated with Sylvester’s multidisciplinary research programs, as well as researchers in a wide variety of cancer-related research projects. The facility also provides training to investigators in cytometric applications, cell sorting technologies and data analysis.

The facility is located in a purpose-built space on the seventh floor of the Rosenstiel building and houses state of the art instruments, with highly experienced staff. Mass cytometry services are available now, for the analysis of 30+ heavy metal-conjugated reagents, using a CyTOF Helios system, with an Hyperion imager for the analysis of solid tissues. A Cytek Aurora spectral analysis cytometer also provides the analysis of 64+ fluorescence parameters. An ALS CellCelector enables the automated aseptic transfer of single cells and colonies from culture plates. A dedicated computer server is available for storage, analysis and archiving of data files. 

Contact us

Eric D. Wieder, Ph.D., Director
Email: ewieder@miami.edu

Phone: 305-243-5571

iLab link for Equipment Reservations

Mailing Address 
Rosenstiel Medical Sciences Building,
1600 N.W. 10th Avenue
Room 7147, Locator R-127
Miami, FL 33136

Physical Address 
Rosenstiel Medical Sciences Building, 
1600 N.W. 10th Avenue
Room 7147
Miami, FL 33136

Please contact the Shared Resource for additional information on services provided and details on recharge rates.