The OGSR is located on the 4th floor of the Papanicolaou Cancer Research Building (PAP) and occupies 2,291 sq.ft. of laboratory space and offices. It comprises distinct pre- and post-PCR labs and BSL2 facilities to support rigorous workflows.
10X Genomics
Illumina NextSeq 500 Sequencers
Nano String Gen2 system
Perkin Elmer Zephyr G3 NGS liquid handler PAP 428
RainDrop Source
OGSR Major Instrumentation:
| Instrument | Description |
|---|---|
| Illumina NovaSeq 6000 | Production-scale short read sequencer |
| Illumina NextSeq 500 | Rapid run, short read, next generation sequencers |
| Illumina iSeq 100 | Ultra low throughput sequencer for QC and protocol validation |
| PE Zephyr G3 NGS | Automated liquid handler for NGS library prep |
| 10x Genomics Chromium | Single Cell Controller for multi-omics assays, optimized for gene expression |
| Mission Bio Tapestri | Single Cell Controller for multi-omics assays, optimized for DNA sequencing |
| Nexcelom K2 | Imaging cell counter with 2 channel fluorescence |
| Agilent Fragment Analyzer 5200 | 12 capillary high-throughput DNA/RNA fragment analyzer |
| Agilent BioAnalyzer 2100 | Lab-on-chip DNA/RNA fragment analysis |
| Thermo Qubit 3.0 | Single tube fluorimeter for dye-based DNA/RNA quantification |
| BioTek Synergy H1 | Multi-channel fluorescence microplate reader |
| Bio-Rad CFX96 | 96-well qPCR machine for quantitation |
| Bio-Rad C1000 (three) | Thermal cyclers with deep-well and standard 96-well blocks |
| Eppendorf MasterCycler | Thermal cycler, 96-well with vapor-protect lid (Pro S model) |
| nanoString Max | nanoString PrepStation and nCounter scanner (2nd generation) |
| Miltenyi GentleMACS | Two rotor homogenizer for tissue dissociation in sealed tubes |
| Covaris CryoPrep CP02 | Cryo-fractionation impactor for 0.05-10 g samples |
| Thermo NanoDrop 8000 | Micro-volume droplet spectrophotometer |
| UVP UV3 HEPA cabs (two) | HEPA filtered UV cabinets for critical workflows |