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Onco-Genomics Technologies

The OGSR is located on the 4th floor of the Papanicolaou Cancer Research Building (PAP) and occupies 660 sq.ft. of laboratory space. It comprises two distinct pre-analytical and analytical facilities: The Sample Processing Laboratory (PAP 428) houses all equipment, liquid handling automation, supplies and resources required for handling and processing samples for nucleic acid extraction, amplification, the preparation gene expression assays and NGS sequencing libraries; The Molecular Analysis Laboratory (PAP 415) houses all of the analytical instrumentation for qPCR, ddPCR, nanoString barcoding/gene expression assays and next generation sequencing. The OGSR office is located in PAP 414.

OGSR Major Instrumentation:

Instrument Description
Illumina NextSeq 500 (two) Rapid run, short read, next generation sequencers
PE Zephyr G3 NGS Automated liquid handler for NGS library prep
10x Genomics Chromium Single Cell Controller for genomics assays
Nexcelom K2 Imaging cell counter with 2 channel fluorescence
AATI Fragment Analyzer 12 capillary high-throughput DNA/RNA fragment analyzer
Agilent BioAnalyzer 2100 Lab-on-chip DNA/RNA fragment analysis
Thermo Qubit 3.0 Single tube fluorimeter for dye-based DNA/RNA quantification
Tecan Infinite F200 Microplate reader, single channel fluorescence
Bio-Rad C1000 (two) Thermal cyclers with deep-well and standard 96-well blocks
Eppendorf MasterCycler Thermal cycler, 96-well with vapor-protect lid (Pro S model)
nanoString Gen2 nanoString PrepStation and nCounter scanner (2nd generation)
RainDance RainDrop Source and Sense instruments for droplet digital PCR
Roche LC480 qPCR 384-well, 2 channel quantitative PCR (qPCR) machine
M├ęcour 40 plate hotel Custom built refrigerated plate hotel for LC480
PE Twister II autoloader Autoloader to for LC480 from plate hotel
Covaris CryoPrep CP02 Cryo-fractionation impactor for 0.05-10 g samples
Thermo NanoDrop 8000 Micro-volume droplet spectrophotometer