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Onco-Genomics Technologies

The OGSR is located on the 4th floor of the Papanicolaou Cancer Research Building (PAP) and occupies 2,291 sq.ft. of laboratory space and offices. It comprises distinct pre- and post-PCR labs and BSL2 facilities to support rigorous workflows.

10X Genomics
Illumina NextSeq 500 Sequencers
Nano String Gen2 system
Perkin Elmer Zephyr G3 NGS liquid handler PAP 428
RainDrop Source

OGSR Major Instrumentation:

Instrument Description
Illumina NovaSeq 6000 Production-scale short read sequencer
Illumina NextSeq 500 Rapid run, short read, next generation sequencers
Illumina iSeq 100 Ultra low throughput sequencer for QC and protocol validation
PE Zephyr G3 NGS Automated liquid handler for NGS library prep
10x Genomics Chromium Single Cell Controller for multi-omics assays, optimized for gene expression
Mission Bio Tapestri Single Cell Controller for multi-omics assays, optimized for DNA sequencing
Nexcelom K2 Imaging cell counter with 2 channel fluorescence
Agilent Fragment Analyzer 5200 12 capillary high-throughput DNA/RNA fragment analyzer
Agilent BioAnalyzer 2100 Lab-on-chip DNA/RNA fragment analysis
Thermo Qubit 3.0 Single tube fluorimeter for dye-based DNA/RNA quantification
BioTek Synergy H1 Multi-channel fluorescence microplate reader
Bio-Rad CFX96 96-well qPCR machine for quantitation
Bio-Rad C1000 (three) Thermal cyclers with deep-well and standard 96-well blocks
Eppendorf MasterCycler Thermal cycler, 96-well with vapor-protect lid (Pro S model)
nanoString Max nanoString PrepStation and nCounter scanner (2nd generation)
Miltenyi GentleMACS Two rotor homogenizer for tissue dissociation in sealed tubes
Covaris CryoPrep CP02 Cryo-fractionation impactor for 0.05-10 g samples
Thermo NanoDrop 8000 Micro-volume droplet spectrophotometer
UVP UV3 HEPA cabs (two) HEPA filtered UV cabinets for critical workflows